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viability  (Dojindo Labs)


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    Structured Review

    Dojindo Labs viability
    Viability, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viability/Cell+Counting+Kit-8/pmc13004575-101-0-8
    Average 99 stars, based on 59726 article reviews
    viability - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Inflammation-responsive biomimetic hybrid nanovesicles reverse stem cell senescence by up-regulating SIRT1 to treat periodontitis.
    Article Snippet: .. Morphology was observed under a microscope at 24, 959 48, and 72 h. Viability was measured using a cell counting kit-8 960 (CCK-8; Dojindo, Tokyo, Japan) by incubating with 10% CCK-8 961 solution at 37°C for 2 h, followed by OD450 absorbance detection 962 with a microplate reader to calculate viability. .. 963 Osteogenic differentiation of PDLSCs in vitro 964 Alkaline Phosphatase (ALP) and Alizarin Red S (ARS) staining 965 Third-generation PDLSCs were seeded at 2×105 cells/well into 966 six-well plates and divided into six groups based on FFA 967 concentration (0, 7.15, 14.31, 21.47, 28.62, or 57.24 μg/mL).

    Article Title: Inflammation-responsive biomimetic hybrid nanovesicles reverse stem cell senescence by up-regulating SIRT1 to treat periodontitis
    Article Snippet: .. Morphology was observed under a microscope at 24, 48, and 72 h. Viability was measured using a cell counting kit-8 (CCK-8; Dojindo, Tokyo, Japan) by incubating with 10% CCK-8 solution at 37 °C for 2 h, followed by OD 450 absorbance detection with a microplate reader to calculate viability. ..

    CCK-8 Assay:

    Article Title: Inflammation-responsive biomimetic hybrid nanovesicles reverse stem cell senescence by up-regulating SIRT1 to treat periodontitis.
    Article Snippet: .. Morphology was observed under a microscope at 24, 959 48, and 72 h. Viability was measured using a cell counting kit-8 960 (CCK-8; Dojindo, Tokyo, Japan) by incubating with 10% CCK-8 961 solution at 37°C for 2 h, followed by OD450 absorbance detection 962 with a microplate reader to calculate viability. .. 963 Osteogenic differentiation of PDLSCs in vitro 964 Alkaline Phosphatase (ALP) and Alizarin Red S (ARS) staining 965 Third-generation PDLSCs were seeded at 2×105 cells/well into 966 six-well plates and divided into six groups based on FFA 967 concentration (0, 7.15, 14.31, 21.47, 28.62, or 57.24 μg/mL).

    Article Title: Inflammation-responsive biomimetic hybrid nanovesicles reverse stem cell senescence by up-regulating SIRT1 to treat periodontitis
    Article Snippet: .. Morphology was observed under a microscope at 24, 48, and 72 h. Viability was measured using a cell counting kit-8 (CCK-8; Dojindo, Tokyo, Japan) by incubating with 10% CCK-8 solution at 37 °C for 2 h, followed by OD 450 absorbance detection with a microplate reader to calculate viability. ..

    Article Title: TRPML3‑mediated lysosomal Ca 2+ release enhances drug sequestration and biogenesis, promoting osimertinib resistance in non‑small cell lung cancer.
    Article Snippet: Images were captured with Mosaic 2.4 software (Tucsen Photonics Co., Ltd.) and analyzed using ImageJ software (version 1.51k; National Institutes of Health) to measure spheroid diameters and other parameters. .. Viability was measured using the Cell Counting Kit‐8 (CCK‐8) assay kit (Dojindo Laboratories, Inc.), according to the manufacturer's protocol. .. In brief, 20 μl of CCK‐8 solution was added to each well and incubated at 37 ̊C for 1 h, after which the absorbance was measured at 450 nm using an iMark microplate reader (Bio‐Rad Laboratories, Inc.).

    Article Title: TRPML3-mediated lysosomal Ca 2+ release enhances drug sequestration and biogenesis, promoting osimertinib resistance in non-small cell lung cancer
    Article Snippet: Images were captured with Mosaic 2.4 software (Tucsen Photonics Co., Ltd.) and analyzed using ImageJ software (version 1.51k; National Institutes of Health) to measure spheroid diameters and other parameters. .. Viability was measured using the Cell Counting Kit-8 (CCK-8) assay kit (Dojindo Laboratories, Inc.), according to the manufacturer's protocol. .. In brief, 20 μl of CCK-8 solution was added to each well and incubated at 37°C for 1 h, after which the absorbance was measured at 450 nm using an iMark microplate reader (Bio-Rad Laboratories, Inc.).

    Article Title: Annexin A1‐Targeted d ‐Peptide‐Monomethyl Auristatin E Conjugate Enhanced Antitumor Effect of Monomethyl Auristatin E for Chemotherapy of Prostate Cancer
    Article Snippet: All flow cytometry data were gated by monodispersed live cells, and histograms of MC16 expression were created by KALUZA V2.1.2 software (Beckman Coulter, CA, USA). .. PC‐3‐hLuc‐PSMA cells were cultured with vcMMAE or c(vcMMAE)dhp7 as vcMMAE concentration ranging from 0 to 12500 ng/100 μL for 48 h, and viability was assessed using Cell counting kit‐8 (Dojindo Laboratory, Kumamoto, Japan). .. The IC50 of each drug conjugate was determined using GraphPad Prism software.

    Co-Culture Assay:

    Article Title: rs10204525 binding to miR-4717-3p modulates PD-1 expression and predicts the development of immune-related adverse events in patients with advanced cancer treated with anti-PD-1/PD-L1 therapy.
    Article Snippet: .. Following a 24 hours or 48 hours of co- culture, HaCaT or BEAS- 2B cells were washed with phosphate- buffered saline (PBS) and their viability was determined by cell counting kit- 8 assay (Dojindo Laboratories, Rockville, Maryland, USA) according to manufacturers’ protocol. .. The absorbance at 450 nm was determined using the Sunrise microplate reader (TECAN, Männedorf, Switzerland).

    Saline:

    Article Title: rs10204525 binding to miR-4717-3p modulates PD-1 expression and predicts the development of immune-related adverse events in patients with advanced cancer treated with anti-PD-1/PD-L1 therapy.
    Article Snippet: .. Following a 24 hours or 48 hours of co- culture, HaCaT or BEAS- 2B cells were washed with phosphate- buffered saline (PBS) and their viability was determined by cell counting kit- 8 assay (Dojindo Laboratories, Rockville, Maryland, USA) according to manufacturers’ protocol. .. The absorbance at 450 nm was determined using the Sunrise microplate reader (TECAN, Männedorf, Switzerland).

    Cell Counting:

    Article Title: rs10204525 binding to miR-4717-3p modulates PD-1 expression and predicts the development of immune-related adverse events in patients with advanced cancer treated with anti-PD-1/PD-L1 therapy.
    Article Snippet: .. Following a 24 hours or 48 hours of co- culture, HaCaT or BEAS- 2B cells were washed with phosphate- buffered saline (PBS) and their viability was determined by cell counting kit- 8 assay (Dojindo Laboratories, Rockville, Maryland, USA) according to manufacturers’ protocol. .. The absorbance at 450 nm was determined using the Sunrise microplate reader (TECAN, Männedorf, Switzerland).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines.
    Article Snippet: .. Viability was measured using cell counting kit 8 (Dojindo), which measures NAD+ turnover as a proxy for viability [21]. ..

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: .. Viability was measured using cell counting kit 8 (Dojindo), which measures NAD+ turnover as a proxy for viability [ ]. ..

    Cell Culture:

    Article Title: Annexin A1‐Targeted d ‐Peptide‐Monomethyl Auristatin E Conjugate Enhanced Antitumor Effect of Monomethyl Auristatin E for Chemotherapy of Prostate Cancer
    Article Snippet: All flow cytometry data were gated by monodispersed live cells, and histograms of MC16 expression were created by KALUZA V2.1.2 software (Beckman Coulter, CA, USA). .. PC‐3‐hLuc‐PSMA cells were cultured with vcMMAE or c(vcMMAE)dhp7 as vcMMAE concentration ranging from 0 to 12500 ng/100 μL for 48 h, and viability was assessed using Cell counting kit‐8 (Dojindo Laboratory, Kumamoto, Japan). .. The IC50 of each drug conjugate was determined using GraphPad Prism software.

    Concentration Assay:

    Article Title: Annexin A1‐Targeted d ‐Peptide‐Monomethyl Auristatin E Conjugate Enhanced Antitumor Effect of Monomethyl Auristatin E for Chemotherapy of Prostate Cancer
    Article Snippet: All flow cytometry data were gated by monodispersed live cells, and histograms of MC16 expression were created by KALUZA V2.1.2 software (Beckman Coulter, CA, USA). .. PC‐3‐hLuc‐PSMA cells were cultured with vcMMAE or c(vcMMAE)dhp7 as vcMMAE concentration ranging from 0 to 12500 ng/100 μL for 48 h, and viability was assessed using Cell counting kit‐8 (Dojindo Laboratory, Kumamoto, Japan). .. The IC50 of each drug conjugate was determined using GraphPad Prism software.



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    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
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    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
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    Image Search Results


    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

    Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control